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This family contains just one protein, SET7/9, which mono-methylates histone at H3K4 92 KMT7 was found to methylate, apart from histone, quite a few non-histone proteins, together with p53, DNA methyltransferase 1 (DNMT1), estrogen receptor alpha (ERα), nuclear factor kappaB (NFκB), and parts of the TATA binding protein (TBP) complicated, TBP-associated elements TAF10 and TAF7 ninety three The consensus recognition sequence in substrates for SET7-mediated lysine methylation, Ok/R-S/T-K (target lysine is daring), can be recognized by the H3K4 demethylase KDM1A/LSD1, which is able to eradicating the methyl mark on most of these substrates 17 , 94 Due to this fact KMT7/KDM1A has emerged as a traditional mannequin for dynamic lysine methylation of both histone and non-histone proteins.
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The members of the SUV39 family discussed above are involved in each euchromatin and heterochromatin, however one other member of the same family, G9a, is the predominant histone H3 K9 methyltransferase in mammalian euchromatin 14 There are two isoforms of G9a within the mouse: the brief type (GenBank accession number NP_671493 ) corresponds to human G9a and the long form ( NP_665829 ), which lacks intron one, has extra Arg-Gly repeats on the amino terminus.
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